6ZWT

Crystal structure of DNA-binding domain of OmpR of two-component system of Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 1
Unit cell lengths	48.470, 49.703, 52.727
Unit cell angles	90.07, 99.01, 103.98

Refinement procedure

Resolution	48.200 - 2.200
R-factor	0.24344
Rwork	0.242
R-free	0.27297
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1opc
RMSD bond length	0.009
RMSD bond angle	1.581
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.150	0.850
Number of reflections	23437	1999
<I/σ(I)>	6.1
Completeness [%]	98.1	97.3
Redundancy	3.6	3.7
CC(1/2)	0.990	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	Bistris propane, Na2SO4, PEG 3350.