6ZW0
Connectase MJ0548 from Methanocaldococcus jannaschii in complex with an MtrA-derived peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.242, 98.500, 109.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.250 - 3.050 |
R-factor | 0.2562 |
Rwork | 0.254 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zvz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.869 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.250 | 49.250 | 3.240 |
High resolution limit [Å] | 3.050 | 9.020 | 3.050 |
Rmerge | 0.144 | 0.031 | 0.993 |
Rmeas | 0.158 | 0.035 | 1.089 |
Number of reflections | 15370 | 656 | 2424 |
<I/σ(I)> | 10.53 | 36.46 | 1.81 |
Completeness [%] | 99.6 | 97.8 | 99.4 |
Redundancy | 6.164 | 5.425 | 5.89 |
CC(1/2) | 0.998 | 0.999 | 0.853 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium cacodylate, 15% PEG 4000 |