6ZVD
14-3-3 Sigma in complex with phosphorylated Gab2pT391 peptide - 96h incubation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-16 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.541 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.350, 111.984, 62.427 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.170 - 2.500 |
R-factor | 0.17739 |
Rwork | 0.174 |
R-free | 0.23364 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lw1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.297 |
Refinement software | REFMAC (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.170 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 10293 | 487 |
<I/σ(I)> | 26.9 | |
Completeness [%] | 100.0 | |
Redundancy | 6.2 | |
CC(1/2) | 0.999 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG400, 1.25% glycerol, 0.2M CaCl, 0.1M HEPES pH 7.5, 2mM BME |