6ZVC

14-3-3 Sigma in complex with phosphorylated Gab2pT391 peptide - 48h incubation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-16
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541
Spacegroup name	C 2 2 21
Unit cell lengths	82.574, 112.508, 62.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.350 - 2.510
R-factor	0.17421
Rwork	0.171
R-free	0.22894
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lw1
RMSD bond length	0.009
RMSD bond angle	1.321
Refinement software	REFMAC (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.350	2.550
High resolution limit [Å]	2.510	2.510
Number of reflections	10297	466
<I/σ(I)>	19
Completeness [%]	99.5
Redundancy	6.1
CC(1/2)	0.997	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG400, 1.25% glycerol, 0.2M CaCl, 0.1M HEPES pH 7.5, 2mM BME