6ZUD
Cu nitrite reductase from Achromobacter cycloclastes: MSOX series at 170K, dose point 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 170 |
| Detector technology | PIXEL |
| Collection date | 2010-07-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 95.355, 95.355, 95.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.960 - 1.100 |
| R-factor | 0.125 |
| Rwork | 0.123 |
| R-free | 0.15500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bw4 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rpim | 0.037 | 0.332 |
| Number of reflections | 115774 | 16660 |
| <I/σ(I)> | 8.5 | 1.9 |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 4.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 293 | 1.6M AMMONIUM SULPHATE, 100MM REMARK 280 SODIUM ACETATE, PH 4.75 |
