6ZTF
Crystal Structure of the anti-human P-Cadherin Fab CQY684
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.999860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.331, 109.384, 139.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.630 - 1.550 |
| R-factor | 0.1889 |
| Rwork | 0.188 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l95 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 69.630 | 69.630 | 1.590 |
| High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
| Rmerge | 0.056 | 0.031 | 0.520 |
| Rmeas | 0.060 | 0.033 | 0.563 |
| Total number of observations | 1035486 | ||
| Number of reflections | 144981 | 1806 | 10620 |
| <I/σ(I)> | 20.56 | 50.26 | 3.52 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 7.142 | 7.065 | 6.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.16M calcium acetate, 14.4% PEG 8,000, 20% glycerol |
