6ZT9
X-ray structure of mutated arabinofuranosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 157.060, 157.060, 379.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 136.020 - 2.000 |
| R-factor | 0.1472 |
| Rwork | 0.147 |
| R-free | 0.17320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vrq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.947 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.020 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 185418 | 13515 |
| <I/σ(I)> | 11.19 | |
| Completeness [%] | 99.9 | |
| Redundancy | 21.71 | |
| CC(1/2) | 0.998 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M ammonium acetate, 0.1 M Bis-tris pH 5.5, 45% (v/v) MPD |
