6ZT8
X-ray structure of mutated arabinofuranosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.999 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 156.630, 156.630, 376.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.000 - 2.800 |
R-factor | 0.2094 |
Rwork | 0.209 |
R-free | 0.24660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vrq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 67863 | 4885 |
<I/σ(I)> | 7.44 | |
Completeness [%] | 99.9 | |
Redundancy | 11.27 | |
CC(1/2) | 0.986 | 0.226 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 34% (v/v) PEG400 |