6ZT7
X-ray structure of mutated arabinofuranosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.969 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 156.790, 156.790, 378.460 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 135.790 - 1.850 |
R-factor | 0.1443 |
Rwork | 0.144 |
R-free | 0.16610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vrq |
RMSD bond length | 0.024 |
RMSD bond angle | 2.162 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 135.790 | 1.900 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 231584 | 16556 |
<I/σ(I)> | 14.17 | |
Completeness [%] | 99.7 | 97.5 |
Redundancy | 19.02 | |
CC(1/2) | 0.998 | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M ammonium acetate, 0.1 M Bis-tris pH 5.5, 45% (v/v) MPD |