6ZT5

Complex between a homodimer of Mycobacterium smegmatis MfpA and a single copy of the N-terminal 47 kDa fragment of the Mycobacterium smegmatis DNA Gyrase B subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	150.545, 59.380, 141.217
Unit cell angles	90.00, 119.71, 90.00

Refinement procedure

Resolution	75.320 - 2.200
R-factor	0.2102
Rwork	0.208
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zt3 6zt4
RMSD bond length	0.007
RMSD bond angle	1.441
Data reduction software	DIALS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.320	75.200	2.260
High resolution limit [Å]	2.200	9.330	2.200
Rmerge	0.048	0.031	1.857
Rmeas	0.052	0.035	2.022
Rpim	0.020	0.014	0.787
Total number of observations		5304	29306
Number of reflections	55380	795	4481
<I/σ(I)>	14.4	66.9	0.8
Completeness [%]	100.0	99.6	100
Redundancy	6.9	6.7	6.5
CC(1/2)	0.996	0.991	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	NULL