6ZSZ
Crystal structure of the Grindelwald extracellular domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 2.0751 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 31.450, 31.450, 97.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.940 - 1.920 |
R-factor | 0.1696 |
Rwork | 0.166 |
R-free | 0.20560 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.880 | 5.210 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.073 | 0.040 |
Number of reflections | 4051 | 89 |
<I/σ(I)> | 163.4 | 11.8 |
Completeness [%] | 96.4 | |
Redundancy | 221.8 | |
CC(1/2) | 1.000 | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Hepes pH 7.5, 45 % MPD, 0.2 M ammonium acetate concentration 82 mg/ml |