6ZSW
M2 mutant (R111K:Y134F:T54V:R132Q:P39Y:R59Y) of human cellular retinoic acid binding protein II - 6 conjugate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.976230 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.192, 58.192, 102.250 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.200 - 2.080 |
R-factor | 0.2079 |
Rwork | 0.207 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yfp |
RMSD bond length | 0.008 |
RMSD bond angle | 1.763 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.200 | 2.190 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.031 | 0.420 |
Rmeas | 0.034 | 0.462 |
Rpim | 0.014 | 0.191 |
Number of reflections | 12539 | 1774 |
<I/σ(I)> | 20 | 2.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.6 | 5.8 |
CC(1/2) | 1.000 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281 | 0.2 M sodium malonate pH 7 and 20 % w/v PEG 3350 |