6ZSV
Structure of crocagin biosynthetic protein CgnB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.972422 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.448, 67.500, 86.152 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 36.170 - 2.300 |
| R-factor | 0.2838 |
| Rwork | 0.283 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zsu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.559 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.170 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 | 0.454 |
| Number of reflections | 25752 | 3773 |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 96.5 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 170 mM ammonium acetate, 85 mM sodium acetate pH 4.6, 25.5% PEG 4000 and 15% glycerol |
