6ZSU
Structure of crocagin biosynthetic protein CgnE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.400, 75.340, 112.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.060 - 2.000 |
R-factor | 0.1585 |
Rwork | 0.156 |
R-free | 0.20180 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.909 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX (1.13_2998) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.060 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.117 | 0.409 |
Number of reflections | 41166 | 2997 |
<I/σ(I)> | 16.9 | 7 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200 mM MgCl2, 100 mM Tris pH 8.5 and 20% PEG 8000 |