6ZSH
The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000010 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.060, 48.190, 100.300 |
Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
Resolution | 44.820 - 2.200 |
R-factor | 0.1796 |
Rwork | 0.177 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d89 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.595 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.820 | 44.820 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.123 | 0.034 | 1.462 |
Rmeas | 0.134 | 0.037 | 1.587 |
Total number of observations | 173618 | ||
Number of reflections | 26307 | 317 | 1939 |
<I/σ(I)> | 11.15 | 31.39 | 2.04 |
Completeness [%] | 99.8 | 96.9 | 99.9 |
Redundancy | 6.6 | 5.486 | 6.74 |
CC(1/2) | 0.998 | 0.999 | 0.712 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M di-Ammonium hydrogen citrate 20% (w/v) PEG 3350 |