6ZS0

Crystal structure of 5-dimethylallyltryptophan synthase from Streptomyces coelicolor

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00002
Spacegroup name	P 1 21 1
Unit cell lengths	44.880, 70.454, 91.145
Unit cell angles	90.00, 92.95, 90.00

Refinement procedure

Resolution	41.055 - 1.500
Rwork	0.189
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5inj
RMSD bond length	0.010
RMSD bond angle	1.425
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (7.1.001)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	1.590
High resolution limit [Å]	1.500	1.500
Rmeas	0.010
Number of reflections	88635	14007
<I/σ(I)>	15.4	1.4
Completeness [%]	97.7
Redundancy	4.6
CC(1/2)	0.990	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	150 mM Bis-tris 23% PEG3350