6ZS0
Crystal structure of 5-dimethylallyltryptophan synthase from Streptomyces coelicolor
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-07 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00002 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.880, 70.454, 91.145 |
Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
Resolution | 41.055 - 1.500 |
Rwork | 0.189 |
R-free | 0.21990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5inj |
RMSD bond length | 0.010 |
RMSD bond angle | 1.425 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (7.1.001) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmeas | 0.010 | |
Number of reflections | 88635 | 14007 |
<I/σ(I)> | 15.4 | 1.4 |
Completeness [%] | 97.7 | |
Redundancy | 4.6 | |
CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 150 mM Bis-tris 23% PEG3350 |