6ZRK
Crystal structure of H8 haemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 98.739, 98.739, 350.383 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 83.070 - 2.000 |
| R-factor | 0.2259 |
| Rwork | 0.224 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.682 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.070 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 44838 | 6458 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.998 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M HEPES pH 7.0, 20-22% PEG3350, 0.2 M lithium citrate |
