6ZRD

STRUCTURE OF THE HUMAN RBAP48 in complex with a macrocyclic peptide cyclized via a xylene linker attached to two cysteines

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97852
Spacegroup name	P 1 21 1
Unit cell lengths	74.400, 59.970, 100.220
Unit cell angles	90.00, 93.83, 90.00

Refinement procedure

Resolution	46.650 - 2.500
R-factor	0.2406
Rwork	0.238
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pbz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.650	46.650	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.227	0.039	2.484
Rmeas	0.246	0.042	2.685
Total number of observations	209413
Number of reflections	30791	376	2258
<I/σ(I)>	7.46	32.05	0.99
Completeness [%]	99.9	98.2	100
Redundancy	6.801	6.436	6.998
CC(1/2)	0.994	0.999	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG3350 0.2M ammonium acetate 0.1M Bis-TRIS pH 5.5