6ZRD
STRUCTURE OF THE HUMAN RBAP48 in complex with a macrocyclic peptide cyclized via a xylene linker attached to two cysteines
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97852 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.400, 59.970, 100.220 |
Unit cell angles | 90.00, 93.83, 90.00 |
Refinement procedure
Resolution | 46.650 - 2.500 |
R-factor | 0.2406 |
Rwork | 0.238 |
R-free | 0.28230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pbz |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18rc7_3834) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.650 | 46.650 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.227 | 0.039 | 2.484 |
Rmeas | 0.246 | 0.042 | 2.685 |
Total number of observations | 209413 | ||
Number of reflections | 30791 | 376 | 2258 |
<I/σ(I)> | 7.46 | 32.05 | 0.99 |
Completeness [%] | 99.9 | 98.2 | 100 |
Redundancy | 6.801 | 6.436 | 6.998 |
CC(1/2) | 0.994 | 0.999 | 0.483 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350 0.2M ammonium acetate 0.1M Bis-TRIS pH 5.5 |