6ZQY
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with Neu5Ac ligand bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 4 |
| Unit cell lengths | 119.290, 119.290, 44.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.720 - 1.850 |
| R-factor | 0.1761 |
| Rwork | 0.175 |
| R-free | 0.19620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m7h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.260 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.720 | 59.640 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.060 | 1.850 |
| Rmerge | 0.076 | 0.025 | 0.388 |
| Rmeas | 0.089 | 0.028 | 0.469 |
| Rpim | 0.046 | 0.014 | 0.258 |
| Total number of observations | 1756 | 9655 | |
| Number of reflections | 52803 | 447 | 3200 |
| <I/σ(I)> | 11.7 | 27.3 | 3 |
| Completeness [%] | 98.6 | 89.5 | 98.7 |
| Redundancy | 3.5 | 3.9 | 3 |
| CC(1/2) | 0.993 | 0.999 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6 M Ammonium Sulphate, 9% Dioxane, 0.1 M MES pH 6.5 |
