6ZQR
Crystal structure of tetrameric fibrinogen-like recognition domain of FIBCD1 with GlcNAc ligand bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 4 |
| Unit cell lengths | 113.600, 113.600, 44.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.930 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4M7H |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.409 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.860 | 56.800 | 1.980 |
| High resolution limit [Å] | 1.930 | 9.050 | 1.930 |
| Rmerge | 0.073 | 0.026 | 0.379 |
| Rmeas | 0.086 | 0.031 | 0.453 |
| Rpim | 0.044 | 0.015 | 0.243 |
| Total number of observations | 1817 | 8913 | |
| Number of reflections | 42095 | 463 | 2759 |
| <I/σ(I)> | 12.4 | 29.7 | 3 |
| Completeness [%] | 98.7 | 99.3 | 98.5 |
| Redundancy | 3.5 | 3.9 | 3.2 |
| CC(1/2) | 0.997 | 0.998 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.5 M Ammonium Sulphate, 7% Dioxane, 0.1 M MES pH 6.5 |
