6ZQ9

Racemic compound of RNA duplexes.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.283
Spacegroup name	P 1
Unit cell lengths	21.000, 25.930, 38.530
Unit cell angles	105.09, 96.63, 91.95

Refinement procedure

Resolution	36.892 - 1.500
Rwork	0.192
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gq6
RMSD bond length	0.008
RMSD bond angle	1.526
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	1.590
High resolution limit [Å]	1.500	1.500
Rmerge		0.967
Number of reflections	11141	6035
<I/σ(I)>	6.88	0.89
Completeness [%]	89.4	86.3
Redundancy	3.54	3.51
CC(1/2)	0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.2 M zinc acetate, 0.1 M cacodylate buffer, 18% w/v polyethylene glycol 8000