6ZPY
Structure of Arabinose-Bound MgGH51 a-L-Arabinofuranosidase Crystal Type 1
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9119 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.708, 65.043, 174.122 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.544 - 1.270 |
| Rwork | 0.139 |
| R-free | 0.17880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zps |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.833 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.040 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rpim | 0.040 | 0.374 |
| Number of reflections | 176357 | 8584 |
| <I/σ(I)> | 8.7 | 0.8 |
| Completeness [%] | 98.5 | 97.3 |
| Redundancy | 7.9 | 7.3 |
| CC(1/2) | 0.996 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10 mg/mL MgGH51 in 10 mM NaOAc, pH 5.5, 100 mM NaCl mixed 2:1 with 20% PEG 3350, 0.1 M Bis-Tris-HCl, pH 6.5, 0.2 M NaNO3 |
