6ZPW
Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 2
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-14 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.953, 125.838, 161.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.743 - 1.329 |
| Rwork | 0.130 |
| R-free | 0.15110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zps |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.931 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.743 | 1.350 |
| High resolution limit [Å] | 1.329 | 1.330 |
| Rpim | 0.035 | 0.727 |
| Number of reflections | 265496 | 12835 |
| <I/σ(I)> | 9.6 | 1.1 |
| Completeness [%] | 99.9 | 98 |
| Redundancy | 8 | 6.8 |
| CC(1/2) | 0.999 | 0.521 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mg/mL MgGH51 in 10 mM NaOAc pH 5.5, 100 mM NaCl mixed 2:1 with 2.4 M (NH4)2SO4, 0.1 M NaOAc, pH 6, 20% glycerol |
