6ZPV
Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-12-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.3775 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.953, 83.953, 256.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.918 - 1.200 |
| Rwork | 0.120 |
| R-free | 0.14400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zps |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.073 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.530 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rpim | 0.011 | 0.430 |
| Number of reflections | 285059 | 13929 |
| <I/σ(I)> | 27.2 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 33 | 25.1 |
| CC(1/2) | 1.000 | 0.421 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mg/mL MgGH51 in 10 mM NaOAc pH 5.5, 100 mM NaCl mixed 2:1 with 1.8 M (NH4)2SO4, 0.1 M NaOAc, pH 5-6, 35% glycerol |
