6ZPS
Structure of Unliganded MgGH51 a-L-Arabinofuranosidase Crystal Type 3 Collected at 2.75 A
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I23 |
Synchrotron site | Diamond |
Beamline | I23 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-12-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 2.755 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.171, 84.171, 257.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.128 - 1.795 |
Rwork | 0.144 |
R-free | 0.17840 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.792 |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 257.350 | 1.800 |
High resolution limit [Å] | 1.790 | 1.790 |
Rpim | 0.012 | 0.142 |
Number of reflections | 74070 | 4624 |
<I/σ(I)> | 35.1 | 5.7 |
Completeness [%] | 84.4 | 90.5 |
Redundancy | 70.2 | 21.5 |
CC(1/2) | 0.999 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mg/mL MgGH51 in 10 mM NaOAc pH 5.5, 100 mM NaCl mixed 2:1 with 1.8 M (NH4)2SO4, 0.1 M NaOAc, pH 5-6, 35% glycerol |