6ZPM

Crystal structure of the unconventional kinetochore protein Trypanosoma cruzi KKT4 coiled coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.976
Spacegroup name	P 1 21 1
Unit cell lengths	33.620, 25.310, 136.880
Unit cell angles	90.00, 96.81, 90.00

Refinement procedure

Resolution	67.960 - 1.900
R-factor	0.2429
Rwork	0.241
R-free	0.25830
Structure solution method	AB INITIO PHASING
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.960	3.880
High resolution limit [Å]	1.900	1.900
Rmerge	0.095
Rmeas	0.103
Rpim	0.040
Number of reflections	16787	1452
<I/σ(I)>	6.1
Completeness [%]	90.4
Redundancy	6.1
CC(1/2)	0.998	0.274

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	HEPES, sodium chloride, MOPSO, Bis-Tris, DL-Arginine hydrochloride, DL- Threonine, DL-Histidine monohydrochloride monohydrate, DL-5-Hydroxylysine hydrochloride, trans-4-hydroxy-L-proline, PEG 8000, 1,5- Pentanediol, TCEP