6ZPK

Crystal structure of the unconventional kinetochore protein Trypanosoma brucei KKT4 BRCT domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	46.368, 61.633, 67.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.600 - 1.570
R-factor	0.1741
Rwork	0.173
R-free	0.19220
Structure solution method	AB INITIO PHASING
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	Arcimboldo
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.601	1.600
High resolution limit [Å]	1.570	1.570
Rmerge		0.549
Rmeas		0.641
Rpim		0.319
Number of reflections	27084	1025
<I/σ(I)>	14
Completeness [%]	97.3
Redundancy	10
CC(1/2)	0.997	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	HEPES, sodium chloride, bis-Tris, ammonium sulphate, TCEP