6ZPJ

Crystal structure of the unconventional kinetochore protein Leishmania mexicana KKT4 coiled coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91587
Spacegroup name	P 1 21 1
Unit cell lengths	31.310, 37.710, 122.390
Unit cell angles	90.00, 92.17, 90.00

Refinement procedure

Resolution	61.150 - 1.900
R-factor	0.2001
Rwork	0.199
R-free	0.23180
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.333
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.151	4.322
High resolution limit [Å]	1.900	1.900
Number of reflections	22522	1840
<I/σ(I)>	10.1
Completeness [%]	98.3	96.5
Redundancy	6.5
CC(1/2)	0.997	0.352

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	HEPES, sodium chloride, imidazole, MPD, TCEP