6ZNS
Crystal Structure of DUF1998 helicase MrfA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | H 3 |
| Unit cell lengths | 191.943, 191.943, 65.892 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.990 - 3.320 |
| R-factor | 0.309 |
| Rwork | 0.307 |
| R-free | 0.34220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292109739 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.527 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.310 | 3.320 |
| Number of reflections | 13373 | 2132 |
| <I/σ(I)> | 11.04 | 0.98 |
| Completeness [%] | 99.8 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.998 | 0.362 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris-HCl, pH 7.5, 0.2 M MgCl2, 10 % (w/v) PEG 4,000 |
