6ZNQ

Crystal Structure of DUF1998 helicase MrfA bound to DNA and AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.918400
Spacegroup name	P 41 21 2
Unit cell lengths	140.570, 140.570, 210.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.730 - 3.340
R-factor	0.2258
Rwork	0.223
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1292109739
RMSD bond length	0.005
RMSD bond angle	0.854
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.540
High resolution limit [Å]	3.340	3.340
Number of reflections	31095	4895
<I/σ(I)>	8.45
Completeness [%]	99.2
Redundancy	14.8
CC(1/2)	0.993	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.24 M tri-sodium citrate, 24 % (w/v) PEG 3350