6ZNQ
Crystal Structure of DUF1998 helicase MrfA bound to DNA and AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-26 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 140.570, 140.570, 210.066 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.730 - 3.340 |
R-factor | 0.2258 |
Rwork | 0.223 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292109739 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.854 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.540 |
High resolution limit [Å] | 3.340 | 3.340 |
Number of reflections | 31095 | 4895 |
<I/σ(I)> | 8.45 | |
Completeness [%] | 99.2 | |
Redundancy | 14.8 | |
CC(1/2) | 0.993 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.24 M tri-sodium citrate, 24 % (w/v) PEG 3350 |