6ZN9

MaeB PTA domain apoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	139.215, 151.278, 285.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.637 - 2.720
Rwork	0.252
R-free	0.29900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1td9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	142.660	2.760
High resolution limit [Å]	2.720	2.720
Number of reflections	162146	7836
<I/σ(I)>	11.4
Completeness [%]	99.9
Redundancy	12.84
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	289	0.1M Sodium Acetate pH 4.5 0.1M Magnesium Acetate 8% PEG 8000