6ZN9
MaeB PTA domain apoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 139.215, 151.278, 285.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.637 - 2.720 |
Rwork | 0.252 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1td9 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.660 | 2.760 |
High resolution limit [Å] | 2.720 | 2.720 |
Number of reflections | 162146 | 7836 |
<I/σ(I)> | 11.4 | |
Completeness [%] | 99.9 | |
Redundancy | 12.84 | |
CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 289 | 0.1M Sodium Acetate pH 4.5 0.1M Magnesium Acetate 8% PEG 8000 |