6ZMQ

Cytochrome c Heme Lyase CcmF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	68.720, 128.270, 134.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.150 - 2.670
R-factor	0.2726
Rwork	0.271
R-free	0.30850
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.980	3.060
High resolution limit [Å]	2.670	2.670
Number of reflections	15589	779
<I/σ(I)>	12.2
Completeness [%]	91.4	58.9
Redundancy	26.8	23.5
CC(1/2)	1.000	0.502

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	18-19 % (v/v) low molecular weight polyethylene glycols (PEG 400, PEG 550 MME, PEG 600, PEG 1000), 0.1 M BisTris pH 6.5, 150 mM MgCl2, 4 % (v/v) trifluorethanol