6ZMP
Crystal structure of Chaetomium thermophilum Naa20 in complex with a bisubstrate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976247 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.150, 114.453, 47.416 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 47.420 - 1.570 |
R-factor | 0.1731 |
Rwork | 0.171 |
R-free | 0.20530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k18 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.420 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Rmerge | 0.087 | 1.306 |
Rpim | 0.056 | 0.817 |
Number of reflections | 67562 | 3320 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 98.7 | 98.4 |
Redundancy | 6.6 | 6.9 |
CC(1/2) | 0.998 | 0.605 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | CtNaa20 was concentrated to 20 mg/ml and mixed in a 1:3 molar ratio with CoA-Ac-MDEL. The crystallization drops contained 200 nl protein solution and 200 nl precipitant solution (15 % (v/v) propanol, 0.2 M ammonium acetate and 0.1 M TRIS pH 8.5). |