6ZMC
Structure of the tRNA-Monooxygenase enzyme MiaE frozen under 2000 bar using the high pressure freezing method
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.770 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.388, 51.909, 78.978 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 47.520 - 2.500 |
| R-factor | 0.1849 |
| Rwork | 0.182 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2itb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.443 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.065 | 0.790 |
| Number of reflections | 16699 | 2618 |
| <I/σ(I)> | 16.5 | 2.24 |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 6.2 | 6.01 |
| CC(1/2) | 0.999 | 0.912 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | protein : 20 mg/ml in 100 mM HEPES, pH 7.5, 30 mM NaCl reservoir : 0.5 M CaCl2, 42% PEG 6k, 2 M Tris-Cl pH 8 |
