Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2019-07-19 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.45883 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 49.000, 49.000, 116.241 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.435 - 1.471 |
| Rwork | 0.217 |
| R-free | 0.24450 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.332 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.440 | 1.560 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmeas | 0.042 | 0.317 |
| Number of reflections | 27246 | 3454 |
| <I/σ(I)> | 25.9 | 0.56 |
| Completeness [%] | 96.1 | 76.1 |
| Redundancy | 16 | 7.5 |
| CC(1/2) | 1.000 | 0.292 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | protein 10-11 mg/ml, 20 mM HEPES pH 8, 200 mM NaCl. reservoir 100 mM imidazole pH 6.5, 1.5 M NaCl, 15 % w/v PEG 3350, 100 mM MgCl2. Cryoprotection Parabar 10312 (Hampton Research) |
