6ZLC

Non-specific dsRNA recognition by wildtype H7N1 RNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98010
Spacegroup name	P 41 21 2
Unit cell lengths	103.596, 103.596, 102.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.330 - 2.300
R-factor	0.1923
Rwork	0.191
R-free	0.22080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zko
RMSD bond length	0.004
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.330	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.086
Number of reflections	25358	2431
<I/σ(I)>	18.8	1.1
Completeness [%]	100.0	100
Redundancy	13.3
CC(1/2)	0.990	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200mM ammonium nitrate, 2M ammonium sulfate