6ZK3

Plant nucleoside hydrolase - ZmNRh2b in complex with ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	94.810, 86.710, 131.910
Unit cell angles	90.00, 99.44, 90.00

Refinement procedure

Resolution	46.500 - 1.750
R-factor	0.162
Rwork	0.161
R-free	0.18100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpo
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.240	1.850
High resolution limit [Å]	1.750	1.750
Rmerge	0.073
Number of reflections	212201	33219
<I/σ(I)>	16
Completeness [%]	99.4
Redundancy	5.6
CC(1/2)	0.999	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	292	PEG 4000, Tris