6ZJX

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant D207A in complex with saccharose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9814
Spacegroup name	P 31 2 1
Unit cell lengths	137.574, 137.574, 126.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.621 - 2.206
R-factor	0.1944
Rwork	0.194
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.003
RMSD bond angle	0.630
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.621	46.621	2.340
High resolution limit [Å]	2.206	6.560	2.210
Rmerge	0.141	0.040	1.598
Rmeas	0.148	0.043	1.687
Number of reflections	69830	2829	11062
<I/σ(I)>	12.58	48.17	1.28
Completeness [%]	99.8	99.6	98.9
Redundancy	10.036	9.334	9.692
CC(1/2)	0.998	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	37% TacsimateTM pH 7.0