6ZJX
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant D207A in complex with saccharose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9814 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.574, 137.574, 126.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.621 - 2.206 |
R-factor | 0.1944 |
Rwork | 0.194 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.630 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.621 | 46.621 | 2.340 |
High resolution limit [Å] | 2.206 | 6.560 | 2.210 |
Rmerge | 0.141 | 0.040 | 1.598 |
Rmeas | 0.148 | 0.043 | 1.687 |
Number of reflections | 69830 | 2829 | 11062 |
<I/σ(I)> | 12.58 | 48.17 | 1.28 |
Completeness [%] | 99.8 | 99.6 | 98.9 |
Redundancy | 10.036 | 9.334 | 9.692 |
CC(1/2) | 0.998 | 0.999 | 0.746 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 37% TacsimateTM pH 7.0 |