6ZJW

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant D207A in complex with galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9814
Spacegroup name	P 31 2 1
Unit cell lengths	139.284, 139.284, 127.816
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.863 - 2.119
R-factor	0.2234
Rwork	0.223
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.003
RMSD bond angle	0.599
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.863	43.863	2.240
High resolution limit [Å]	2.110	6.280	2.110
Rmerge	0.180	0.069	1.806
Rmeas	0.199	0.077	2.006
Total number of observations	441940
Number of reflections	81346	3291	12437
<I/σ(I)>	5.18	19.08	0.54
Completeness [%]	98.8	98.7	94.3
Redundancy	5.433	5.162	5.214
CC(1/2)	0.993	0.995	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	37% TacsimateTM pH 8.0