6ZJW
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant D207A in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9814 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 139.284, 139.284, 127.816 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.863 - 2.119 |
R-factor | 0.2234 |
Rwork | 0.223 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.003 |
RMSD bond angle | 0.599 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.863 | 43.863 | 2.240 |
High resolution limit [Å] | 2.110 | 6.280 | 2.110 |
Rmerge | 0.180 | 0.069 | 1.806 |
Rmeas | 0.199 | 0.077 | 2.006 |
Total number of observations | 441940 | ||
Number of reflections | 81346 | 3291 | 12437 |
<I/σ(I)> | 5.18 | 19.08 | 0.54 |
Completeness [%] | 98.8 | 98.7 | 94.3 |
Redundancy | 5.433 | 5.162 | 5.214 |
CC(1/2) | 0.993 | 0.995 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 37% TacsimateTM pH 8.0 |