6ZJV

Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant D207A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9814
Spacegroup name	P 31 2 1
Unit cell lengths	139.451, 139.451, 127.877
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.125 - 2.250
R-factor	0.2365
Rwork	0.236
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6etz
RMSD bond length	0.004
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.125	47.125	2.380
High resolution limit [Å]	2.246	6.680	2.250
Rmerge	0.111	0.034	1.801
Rmeas	0.114	0.035	1.848
Number of reflections	68473	2779	10818
<I/σ(I)>	21.38	84.82	1.52
Completeness [%]	99.6	99.4	98.7
Redundancy	20.028	17.982	19.95
CC(1/2)	1.000	1.000	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	28% TacsimateTM pH 7.5