6ZJU
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E441Q in complex with saccharose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9814 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 138.241, 138.241, 127.568 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.649 - 1.750 |
R-factor | 0.1697 |
Rwork | 0.170 |
R-free | 0.18180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.083 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.649 | 43.649 | 1.860 |
High resolution limit [Å] | 1.750 | 5.220 | 1.750 |
Rmerge | 0.129 | 0.026 | 4.034 |
Rmeas | 0.136 | 0.027 | 4.256 |
Total number of observations | 1425743 | ||
Number of reflections | 141154 | 5597 | 22540 |
<I/σ(I)> | 14.42 | 74.66 | 0.56 |
Completeness [%] | 99.9 | 99.8 | 99.7 |
Redundancy | 10.101 | 9.624 | 9.825 |
CC(1/2) | 0.999 | 1.000 | 0.322 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 35% TacsimateTM pH 7.0 |