6ZJR
Cold-adapted beta-D-galactosidase from Arthrobacter sp. 32cB mutant E517Q in complex with lactulose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9814 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.427, 137.427, 127.253 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.686 - 2.000 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6etz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.004 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.686 | 46.686 | 2.120 |
High resolution limit [Å] | 1.998 | 5.950 | 2.000 |
Rmerge | 0.148 | 0.033 | 2.438 |
Rmeas | 0.156 | 0.035 | 2.577 |
Number of reflections | 94041 | 3786 | 14819 |
<I/σ(I)> | 12.54 | 57.48 | 0.78 |
Completeness [%] | 99.7 | 99.7 | 98.4 |
Redundancy | 10.019 | 9.703 | 9.494 |
CC(1/2) | 0.999 | 0.999 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 273 | 37% TacsimateTM pH 8.0 |