6ZJK
Ribonucleotide reductase R2 subunit from Clostridium botulinum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 115 |
| Detector technology | PIXEL |
| Collection date | 2016-07-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.827, 61.854, 154.342 |
| Unit cell angles | 90.00, 127.46, 90.00 |
Refinement procedure
| Resolution | 83.670 - 2.000 |
| R-factor | 0.2227 |
| Rwork | 0.222 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcc |
| Data reduction software | DIALS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc4-3812-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.680 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | |
| Number of reflections | 106664 | 7815 |
| <I/σ(I)> | 10.71 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | sodium bromide, Bis Tris propane pH 6.5, PEG 3350 |
