6ZJ2
Structure of RcsB from Salmonella enterica serovar Typhimurium bound to promoter rprA in the presence of phosphomimetic BeF3-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.356, 111.514, 146.262 |
| Unit cell angles | 90.00, 90.36, 90.00 |
Refinement procedure
| Resolution | 45.990 - 3.380 |
| R-factor | 0.2681 |
| Rwork | 0.266 |
| R-free | 0.31860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5o8z |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.990 | 45.990 | 3.580 |
| High resolution limit [Å] | 3.380 | 10.750 | 3.400 |
| Rmerge | 0.228 | 0.120 | 0.654 |
| Rmeas | 0.266 | 0.139 | 0.771 |
| Rpim | 0.133 | 0.069 | 0.398 |
| Total number of observations | 3622 | 15807 | |
| Number of reflections | 30572 | 1009 | 4382 |
| <I/σ(I)> | 4.2 | 7.9 | 1.9 |
| Completeness [%] | 99.1 | 98.3 | 97.8 |
| Redundancy | 3.8 | 3.6 | 3.6 |
| CC(1/2) | 0.983 | 0.989 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 15% PEG 8000, MES pH 6.5 and 0.2M NaAc |
