6ZIV
Crystal structure of a Beta-glucosidase from Alicyclobacillus acidiphilus
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9139 |
Spacegroup name | P 1 |
Unit cell lengths | 62.654, 91.644, 159.342 |
Unit cell angles | 91.62, 90.42, 89.99 |
Refinement procedure
Resolution | 46.550 - 1.950 |
Rwork | 0.262 |
R-free | 0.29630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | sequence |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 159.274 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.098 | 0.561 |
Number of reflections | 367007 | 18349 |
<I/σ(I)> | 9 | |
Completeness [%] | 91.1 | |
Redundancy | 84.9 | |
CC(1/2) | 0.996 | 0.608 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | condition H3 (0.1 M Bis-Tris pH 5.5 and 25% (w/v) PEG3350) from the JCSG screen (Molecular Dimensions) |