6ZIJ

Crystal Structure of Two-Domain Laccase mutant R240H from Streptomyces griseoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	75.040, 94.320, 116.830
Unit cell angles	90.00, 90.69, 90.00

Refinement procedure

Resolution	45.830 - 1.600
R-factor	0.1466
Rwork	0.145
R-free	0.18110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lhl
RMSD bond length	0.007
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.090	0.970
Number of reflections	207262	15082
<I/σ(I)>	7.34	1.13
Completeness [%]	96.8
Redundancy	3.34
CC(1/2)	0.990	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	20 % w/v PEG 3350 0.2 M Ammonium chloride