6ZIF
The structure of a cytosolic copper storage protein from Methylocystis sp. Strain Rockwell (ATCC 49242)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96878 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 60.760, 60.760, 231.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.770 - 2.200 |
R-factor | 0.21362 |
Rwork | 0.211 |
R-free | 0.27036 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lmf |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.772 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.174 | |
Rmeas | 0.190 | |
Rpim | 0.074 | |
Number of reflections | 22975 | 1957 |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.3 | |
Redundancy | 11.7 | 12.3 |
CC(1/2) | 0.997 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM MgCl2, 100 mM Hepes pH 7.5 and 25% PEG 3350 |