6ZI3

Crystal structure of OleP-6DEB bound to L-rhamnose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	247.379, 111.149, 159.144
Unit cell angles	90.00, 129.40, 90.00

Refinement procedure

Resolution	48.090 - 2.080
R-factor	0.17438
Rwork	0.172
R-free	0.22501
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5mns
RMSD bond length	0.011
RMSD bond angle	1.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	1.960	1.960
Rmerge	0.064	1.640
Number of reflections	462663	73872
<I/σ(I)>	7.8
Completeness [%]	97.7
Redundancy	2.7
CC(1/2)	0.997	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	4.2 M Sodium Formate (HCOONa)