6ZHG
Ca2+-ATPase from Listeria Monocytogenes in complex with AlF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-07-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 181.272, 69.213, 124.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.470 - 4.000 |
| R-factor | 0.3094 |
| Rwork | 0.308 |
| R-free | 0.32690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zhh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.028 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.200 | 4.200 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 13420 | 1811 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 97.6 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.996 | 0.333 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 16% PEG6000, 14% glycerol, 0.11M MgCl2, 3.75% t-butanol, 5mM BMe and 80-160mM Z3-10 |
