6ZHF
Calcium ATPase-1 from Listeria monocytogenes in complex with BeF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.050, 143.857, 153.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.391 - 4.000 |
| R-factor | 0.2343 |
| Rwork | 0.232 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zhh |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 4.200 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 13285 | 1772 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 98.4 | |
| Redundancy | 3 | |
| CC(1/2) | 0.989 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 7% PEG6000, 3% t-BuOH, 100mM LiSO4, 5mM BME, 100mM KCl, 19mM C8E4 |
